The case of the French botanist Philippe Van Tieghem (1839-1914) allows retracing the development of the approach of plant tissues culture in the second half of the nineteenth century. In his « Physiological researches on germination » (1873) in particular, this biologist offers apparently to study, through mutilations (by disrupting embryonic development), all correlations (or mutual influences) of embryonic organs in plant organism, that is to say, the properties and potentialities of plant embryonic parts themselves and in the embryo. Beyond the experimental achievements to study plant morphology and physiology, these researches and ideas led this botanist to formulate hypotheses and set forth the practical and theoretical perspectives hiring the concept of cell.
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